Acid Fast Bacteria Stain Kit from American MasterTech

Stains for mycobacteria (acid fast bacteria) in less than 15 minutes!

Acid-fast techniques are useful in identifying acid-fast organisms, mainly mycobacteria. The most common types of mycobacteria found are Mycobacterium tuberculosis, which is responsible for tuberculosis (TB), and Mycobacterium leprae which is responsible for leprosy. Acid-fastness describes the physical property of certain bacteria's resistance to decolorization by dilute acids and/or alcohol based decolorization solutions. Acid-fast organisms are difficult to stain using standard stains, such as Gram's stain, due to the large amounts of lipids on the acid-fast organism's wall that resist these types of stain.

In 1882 Franz Ziehl and Friedrich Neelsen demonstrated that certain bacteria stained with a hot phenol-fuchsin solution resisted decolorization with acid alcohol; these orgranisms were identified as acid-fast. Then in 1915, JJ Kinyoun modified the Ziehl-Neelsen's technique so that the heating of the phenol-fuchsin solution was no longer required. During the past century, the Ziehl-Neelsen and Kinyoun methods have been modified by many practioners, but the basic process remains relevant today.

Interested in learning more about Acid Fast Bacteria? Here is an article that explains why American MasterTech's Acid Fast Bacteria Stain Kit is the better choice for your lab. "Methylene Blues" was written by our own QC department and was featured in the Fall/Winter 2009 MasterTech Flyer.

MasterTech's Acid Fast Bacteria Stain Kit
American MasterTech's Acid Fast Bacteria Stain Kitis suitable, in lieu of and comparable to the Kinyoun's AFB and Ziehl-Neelsen procedures for staining all mycobacteria, including Tubercle Bacillus. The MasterTech AFB Stain Kit differs from those procedures in that Advanced Carbol Fuchsin™ (ACF) is used for bacterial staining instead of a standard Carbol Fuchsin, and Light Green is used as a counterstain instead of Methylene Blue. In comparison to standard Carbol Fuchsins, MasterTech Acid Fast Bacteria Stain Kit requires no heat and much shorter staining times by combining three types of fuchsin dyes. Light Green is a much better counterstain than Methylene Blue, as it provides higher contrast against the red stained bacteria.

Acid Fast Bacteria Stain Kit The Acid Fast Bacteria Stain Kit is available in these size(s):100ml or Pint Kit. Acid Fast Bacteria Stain Kit Procedure

MasterTech's Acid Fast Bacteria - Modified Truant's Fluorescent Stain Kit
For labs with a fluorescence capable microscope, the Acid Fast Bacteria Modified Truant's Fluorescent Stain Kit provides a much greater contrast than standard light microscope procedures. The Acid Fast Bacteria Modified Truant's Fluorescent Stain Kit stains for mycobacteria (acid fast bacteria) in as little as 10 minutes. Auramine O-Rhodamine B serves as the primary stain, producing fluorescent yellow to orange bacteria against a black background produced by Eriochrome Black T.

Acid Fast Bacteria Stain Kit - Modified Truant's Fluorescent The Acid Fast Bacteria Stain Kit is available in these size(s): 100ml Kit AFB Truant's Fluorescent Stain Kit Procedure

Acid Fast Bacteria Control Slides
Don't forget to pick up a pack of Acid Fast Bacteria Control Slides to complete the stain! Each MasterTech Acid Fast Bacteria Special Stain control slide is prepared using top quality tissue that has been processed with the utmost care. After processing, the tissue is carefully embedded in paraffin blocks, cut at an optimal thickness, and adhered to the central region of a superfrost slide. Slides are packaged safely in durable plastic containers. 5 packs and 25 packs are all unstained. Controls also available with 1 Stained QC Slide and 24 Unstained Slides.

Acid Fast Bacteria Control Slides The Acid Fast Bacteria Control Slides are available in these size(s): 5 Pack, 25 Pack, and QC Pack