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Just like in light microscopy, the most important step in electron microscopy is tissue fixation. During this process, the piece of tissue is placed into a fixative within 15 minutes of excision to avoid autolysis. If the correct fixative is used, the plasmalemma, nuclear envelope, mitochondria, endoplasmic reticulum, cytoplasm, and nucleus can be visible using an electron microscope. Tissue fixation using common histological fixatives does not penetrate rapidly enough into the cellular level and does not preserve the tissue sufficiently for electron microscopy. Therefore, the correct fixative must be used to achieve optimal results.
As a popular dual-purpose fixative used in both electron microscopy and light microscopy, Millonig's Fixative is a combination of formaldehyde, water, sodium phosphate, and sodium hydroxide with a pH of 7.2 to 7.4. American MasterTech's Millonig's Fixative (based on Carson's modification of Millonig's original method) penetrates tissue quickly, optimally preserves it, and is indefinitely stable at room temperature, making it an ideal fixative for electron microscopy. Unlike other fixatives used in electron microscopy that allow tissue to be fixed no longer then a few hours, Millonig's Fixative allows tissue to remain in the fixative indefinitely, allowing electron microscopy to be done anytime.
Whether you use Millonig's Fixative for electron or light microscopy, American MasterTech offers high-quality Millonig's Fixative at a great price. American MasterTech's Millonig's Fixative is available in a pint sized bottle.