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Van Gieson’s Stain has been proven the world over as the easiest method of differential staining of collagen and other connective tissue. A mixture of Picric Acid and Acid Fuchsin, Van Gieson’s Stain has been around since 1889, when it was first introduced by Ira Van Gieson. Van Gieson's Stain is just one of many things Dr. Van Gieson accomplished before succumbing to chronic nephritis at the age of 47.
When stained with the MasterTech Van Gieson’s Stain, collagen will stain pink or deep red, and cytoplasm will be stained yellow. Often times, Van Gieson's is used as a counterstain to other methods. In these instances, nuclear staining might not be neccessary.
Van Gieson's Stain is also widely used when staining for Elastic Fibers in tissues such as skin or aorta, via the Verhoeff Method. The elastic fibers will be stained blue to black and background will be stained yellow. For excellent Elastic Fiber staining, try the
Verhoeff's Elastic Stain Kit from American MasterTech.
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