Von Kossa has been the most popular method for indirectly identifying the presence of calcium in tissue since 1901. The Von Kossa procedure does not stain for calcium itself, but instead it stains calcium deposits or salts. The Von Kossa method used today has been modified from its original version by replacing the higher silver nitrate concentration that was used in the original Von Kossa method with 5% silver nitrate solution, which resulted in greater sensitivity in staining.
The Von Kossa method is a metal substitution technique in which the metal (silver nitrate solution) is used to transform calcium salts into metal salts. In the Von Kossa method, the silver nitrate solution serves as the primary stain by replacing the phosphate and carbonate anions with silver ions, producing silver salts. The silver salts are then reduced by using a strong, bright light and the unreduced silver is removed by using sodium thiosulfate.
American MasterTech's Von Kossa Stain Kit produces brown to black calcium salts against pink to red cytoplasm and nuclei in just 30 minutes. MasterTech's Von Kossa Stain Kit is available in two convenient sizes: the 100ml Kit, which yields 20-32 slides, or the Pint Kit, which yields 50-192 slides. Each Von Kossa Stain Kit is tamper-evident sealed to ensure quality and includes 5% Silver Nitrate Solution, 5% Sodium Thiosulfate, Nuclear Fast Red Stain, and easy-to-follow instructions.
Website Keywords to aid in Proper Search Engine Placement for Yahoo, Google, Overture, Lycos, Metacrawler, Altavista, and MSN: Laboratory Supplies from American MasterTech, Von Kossa Method, Von Kossa Procedure, Von Kossa Stain, Von Kossa Staining, Von Kossa Calcium Stain, Von Kossa Staining for Calcium, Von Kossa Stain Kit, Von Kossa technique, Silver Nitrate Solution, Sodium Thiosulfate, Calcium Salts, Calcium Deposits, Phosphate Calcium, Carbonate Calcium.
Von Kossa has been the most popular method for indirectly identifying the presence of calcium in tissue since 1901. The Von Kossa procedure does not stain for calcium itself, but instead it stains calcium deposits or salts. The Von Kossa method used today has been modified from its original version by replacing the higher silver nitrate concentration that was used in the original Von Kossa method with 5% silver nitrate solution, which resulted in greater sensitivity in staining.
