Cytomegalovirus belongs to the ß-herpes virus family and globally affects the world population. Primary infection is often a flulike process although there are known cases of greater severity that are clinically similar to mononucleosis.
Like other herpes viruses, after primary infection CMV persists in the host permanently as a latent infection.
Immunosuppression in patients can provoke reactivation of the virus giving rise to serious lesions in a multitude of organs such as the lungs, kidneys, central nervous system, gastrointestinal apparatus, endothelium and blood cells.
After infection, CMV first begins to transcribe the so-called ‘early’ genes. Of these genes, one known as ß2.7 (2.7 kbases) is the most abundantly transcribed and accumulates during the entire infection until it makes up 20% of all mRNA transcribed by the virus. Because of this, the gene ß2.7 is an ideal candidate for CMV identification.
CENBIMO has fabricated HistoSonda® CMV which consists of a single strand of DNA with a sequence length of 288 nucleotides. This sequence is complementary to CMV gene ß2.7 mRNA. The probe DNA has been labeled with digoxigenin.
The technique of in situ hybridization presents numerous advantages over immunohistochemistry and unspecific results obtained when using antibodies can be avoided.
Intended for research use only (RUO).