This enzyme is found in a variety of human cells but principally in tissular histiocytes, circulating granulocytes, monocytes and in glandular saliva and lacrimal cells.
The quantity of lysozyme produced by monocytes is greatly superior to that of polymorphonuclear neutrophil granulocytes. Various point mutations in the lysozyme gene have been associated with non-neuropathic visceral amylodosis.
HistoSonda Lysozyme consists of a fragment of single stranded DNA 358 nucleotides long that is targeted against lysozyme RNA, the principle use of this probe is the classification and study of histiocytic cells (especially when they are activated and functioning) and the detection of monocytes in the bone marrow.
Due to the fact that in decalcified, paraffin embedded bone marrow biopsies it is not possible to clearly see the granulocytic cytoplasmic granuoles it is frequently very difficult to distinguish between myelocytes and monocytes.
HistoSonda Lysozyme permits a clean and clear differentiation of the bone marrow monocytic cells owing to the augmented transcription of the gene producing lysozyme.
This permits the clear differentiation of chronic myelomonocytic leukemia from other types of chronic granulocytic leukemias.
Intended for research use only (RUO).